3-Cyclohexylamino-2-hydroxypropanesulfonic acid sodium salt
[CAS# 102601-34-3]

Identification

• Classification: Organic raw materials >> Amino compound >> Sulfonic acid amino compound
• Name: 3-Cyclohexylamino-2-hydroxypropanesulfonic acid sodium salt
• Synonyms: CAPSO sodium salt
• Molecular Formula: C₉H₁₈NNaO₄S
• Molecular Weight: 259.30
• CAS Registry Number: 102601-34-3
• EC Number: 700-631-8
• SMILES: C1CCC(CC1)NCC(CS(=O)(=O)[O-])O.[Na+]

Properties

• Solubility: 0.143 g/mL (water)

Safety Data

• Hazard Symbols: GHS05 Danger
• Risk Statements: H318
• Safety Statements: P280-P305+P351+P338+P310

Hazard Classification

Hazard	Class	Category Code	Hazard Statement
Serious eye damage	Eye Dam.	1	H318

Discovery and Applications

3-Cyclohexylamino-2-hydroxypropanesulfonic acid sodium salt, commonly referred to as CAPSO-Na, is a zwitterionic buffer used in biochemical and molecular biology applications. CAPSO-Na is part of the Good family of buffers, named after Dr. Norman Good, who in the 1960s discovered several buffers that were well suited for biological research because they minimally interfered with biochemical processes.

CAPSO-Na has a molecular formula of C9H18NNaO4S, combining a cyclohexylamine group with a hydroxypropanesulfonic acid moiety, neutralized by a sodium ion. This structure enables CAPSO-Na to effectively buffer solutions in the pH range of 8.9 to 10.3, making it suitable for applications that require a stable, alkaline pH environment. The presence of the cyclohexyl group enhances the compound's stability and buffering capacity.

CAPSO-Na is widely used in research involving proteins and enzymes that function best under alkaline conditions. Its ability to maintain a stable pH ensures that proteins and enzymes retain their native conformation and activity during experiments.

In gel electrophoresis, CAPSO-Na is used as a buffer for separating nucleic acids and proteins. Its stability in high pH environments makes it particularly useful in systems where traditional buffers fail to provide consistent results.

Although less common than other buffers, CAPSO-Na can be used in specialized cell culture applications where a basic pH needs to be maintained. It helps create a stable environment that supports cell function and viability.

CAPSO-Na is used in a variety of biochemical assays, especially those that require high pH. Its low reactivity with analytical components ensures accurate and reproducible results.

The buffer range offered by CAPSO-Na is ideal for applications that require an alkaline environment, ensuring consistent experimental conditions. The compound's low reactivity with biomolecules reduces the risk of interference and improves the reliability of experimental data. The cyclohexylamine group in CAPSO-Na contributes to its stability, making it a reliable choice for a variety of applications.

References

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DOI: 10.1007/s00203-021-02543-9

2024. Roseobacter weihaiensis sp. nov., a cellulose-degrading bacterium isolated from intestinal content of Nipponacmea schrenckii collected from golden beach in Weihai, China. Antonie van Leeuwenhoek, 117(1).
DOI: 10.1007/s10482-024-02009-0