Chymopapain
[CAS# 9001-09-6]

Identification

• Classification: API >> Synthetic anti-infective drugs >> Natural source anti-infectives
• Name: Chymopapain
• Synonyms: BAX 1526; Chymodiactin; Chymopapain C; Cysteine proteinase CC-III; E.C. 3.4.22.6; E.C. 3.4.4.11; NSC 107079
• CAS Registry Number: 9001-09-6
• EC Number: 232-580-8

Properties

• Solubility: Soluble
• Melting point: 137-182 °C

Safety Data

• Hazard Symbols: GHS07;GHS08 Danger
• Risk Statements: H315-H317-H319-H334-H335
• Safety Statements: P233-P260-P261-P264-P264+P265-P271-P272-P280-P284-P302+P352-P304+P340-P305+P351+P338-P319-P321-P332+P317-P333+P317-P337+P317-P342+P316-P362+P364-P403-P403+P233-P405-P501

Hazard Classification

Hazard	Class	Category Code	Hazard Statement
Skin sensitization	Skin Sens.	1	H317
Eye irritation	Eye Irrit.	2	H319
Skin irritation	Skin Irrit.	2	H315
Respiratory sensitization	Resp. Sens.	1	H334
Specific target organ toxicity - single exposure	STOT SE	3	H335

Discovery and Applications

Chymopapain is a proteolytic enzyme derived from the latex of the papaya fruit (Carica papaya). Its discovery and subsequent applications have been significant in the field of biochemistry and medicine. This enzyme was first identified in the mid-20th century, as researchers explored the properties of plant-derived enzymes and their potential therapeutic uses.

The discovery of chymopapain stemmed from studies on the proteolytic enzymes present in papaya latex. Early research demonstrated that papaya latex contained several enzymes with the ability to break down proteins. Chymopapain, specifically, was distinguished by its unique properties, including its ability to hydrolyze peptide bonds in proteins, making it a valuable tool in various biochemical applications.

In medical applications, chymopapain has been primarily used in the treatment of herniated discs. As a proteolytic enzyme, chymopapain can break down the protein components of the herniated disc material, which helps reduce pressure on the spinal nerves. This application is known as chemonucleolysis, a minimally invasive procedure used to alleviate pain and improve function in patients with lumbar disc herniation. The use of chymopapain in chemonucleolysis has been associated with significant pain relief and improved mobility in many patients.

Chymopapain is also utilized in the preparation of various biochemical assays and research applications. Its ability to selectively degrade proteins makes it a useful reagent for studying protein structure and function. In addition, it is employed in the study of enzyme kinetics and the development of new enzymatic assays. Its application extends to the food industry, where it is used as a meat tenderizer due to its proteolytic activity.

Despite its beneficial uses, the application of chymopapain in medical procedures has been somewhat limited due to potential side effects and the availability of alternative treatments. Concerns about allergic reactions and the potential for adverse effects have led to a decline in its use for chemonucleolysis. Nonetheless, research into its applications and properties continues, contributing to a deeper understanding of proteolytic enzymes and their roles in both health and disease.

Chymopapain's discovery and application highlight the importance of plant-derived enzymes in therapeutic and industrial contexts. Its ability to interact with proteins in specific and useful ways has established it as a valuable tool in both clinical and research settings.

References

1963. Kiso to Rinsho. Clinical Report. Clinical Report, 27.
DOI: None