Rhodamine B
[CAS# 81-88-9]

Identification

• Classification: Organic raw materials >> Organic phosphine compound
• Name: Rhodamine B
• Synonyms: Basic Violet 10; C.I. 45170; 9-(2-Carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
• Molecular Formula: C₂₈H₃₁N₂O₃^.Cl
• Molecular Weight: 479.02
• CAS Registry Number: 81-88-9
• EC Number: 201-383-9
• SMILES: CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC)CC)C=C3O2)C4=CC=CC=C4C(=O)O.[Cl-]

Properties

• Density: 1.31 g/mL
• Melting point: 210-211 ºC (dec.)
• Water solubility: Soluble

Safety Data

• Hazard Symbols: GHS05;GHS07 Danger
• Hazard Statements: H302-H318-H412
• Precautionary Statements: P264-P264+P265-P270-P273-P280-P301+P317-P305+P354+P338-P317-P330-P501

Hazard Classification

Hazard	Class	Category Code	Hazard Statement
Serious eye damage	Eye Dam.	1	H318
Chronic hazardous to the aquatic environment	Aquatic Chronic	3	H412
Acute toxicity	Acute Tox.	4	H302
Specific target organ toxicity - single exposure	STOT SE	2	H371
Acute toxicity	Acute Tox.	4	H332
Acute toxicity	Acute Tox.	4	H312
Germ cell mutagenicity	Muta.	2	H340
Specific target organ toxicity - single exposure	STOT SE	3	H335
Acute toxicity	Acute Tox.	3	H301
Skin irritation	Skin Irrit.	2	H315
Carcinogenicity	Carc.	2	H351
Eye irritation	Eye Irrit.	2	H319
Chronic hazardous to the aquatic environment	Aquatic Chronic	4	H413
Skin sensitization	Skin Sens.	1	H317
Germ cell mutagenicity	Muta.	2	H341

Discovory and Applicatios

Rhodamine B is a synthetic dye that belongs to the rhodamine family and is widely used for its vibrant pink color and fluorescent properties. Discovered in the late 19th century, Rhodamine B was initially synthesized by the German chemist Heinrich Caro in 1883 as a member of the xanthene dye class. The compound is characterized by its molecular formula C28H31ClN2O3 and exhibits strong absorption and emission properties in the visible light spectrum, making it a valuable substance in various applications.

One of the primary applications of Rhodamine B is in the textile industry, where it is used as a dye for fabrics, particularly in cotton and wool processing. Its excellent color fastness and ability to impart bright hues make it a popular choice for dyeing materials. In addition to textiles, Rhodamine B is utilized in the paper industry to enhance the brightness of papers and cardboard products. The compound's fluorescent properties also make it useful in the production of fluorescent markers and inks, which are widely employed in printing and packaging.

Rhodamine B is not only limited to industrial applications; it also has significant uses in the field of biology and medicine. The dye is commonly used as a tracer in biological and environmental studies due to its fluorescent properties, which allow researchers to track the movement of substances in various systems. In particular, Rhodamine B has been utilized in cell labeling and imaging studies, enabling scientists to visualize cellular processes and structures under fluorescence microscopy.

Furthermore, Rhodamine B is frequently employed in analytical chemistry as a fluorescent probe for detecting metal ions, such as lead and copper, in environmental samples. Its sensitivity and specificity for certain metal ions make it a valuable tool for monitoring water quality and environmental pollution.

Despite its widespread use, there are concerns regarding the toxicity of Rhodamine B. Studies have indicated that the dye can exhibit mutagenic and carcinogenic properties under certain conditions, raising safety concerns for both humans and the environment. As a result, regulatory agencies in some countries have imposed restrictions on the use of Rhodamine B in food and cosmetic products, prompting researchers to explore safer alternatives.

In summary, Rhodamine B is a versatile dye with a wide range of applications in textiles, biology, and analytical chemistry. Its discovery in the 19th century has paved the way for various industrial and scientific advancements. However, the potential health and environmental risks associated with its use highlight the need for careful handling and consideration of alternative substances in certain applications.