Ion-pair reagents are vital in HPLC (High-Performance Liquid Chromatography) for separating charged compounds like peptides, nucleotides, and pharmaceuticals. They form ion pairs with analytes, improving retention on the column. Commonly used ion-pairing agents include TFA (trifluoroacetic acid) and HFBA (heptafluorobutyric acid), enhancing HPLC's utility in various analytical and research applications.
