PIPES sesquisodium salt
[CAS# 100037-69-2]

Identification

• Classification: Organic raw materials >> Heterocyclic compound
• Name: PIPES sesquisodium salt
• Synonyms: 1,4-Piperazinediethanesulfonic acid sesquisodium salt; Piperazine-N,N'-bis(2-ethanesulfonic acid) sesquisodium salt
• Molecular Formula: C₁₆H₃₃N₄Na₃O₁₂S₄
• Molecular Weight: 670.66
• CAS Registry Number: 100037-69-2
• EC Number: 600-018-4
• SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+]

Properties

• Solubility: 0.25 g/mL (0.1 M NaOH)
• Melting point: 300 °C

Safety Data

• Hazard Symbols: GHS07 Warning
• Risk Statements: H302-H315-H319-H335
• Safety Statements: P261-P305+P351+P338

Discovery and Applications

PIPES sodium sesquihydrate is a widely used buffer in biochemistry and molecular biology derived from the organic compound PIPES (1,4-piperazinediethanesulfonic acid). This compound was discovered by researchers in the 1960s who were looking for effective zwitterionic buffers for biological systems. PIPES, like other good buffers, has the advantages of low reactivity, stability at different temperatures, and negligible interference with biological processes.

PIPES sodium sesquihydrate has the molecular formula C8H16N2Na1.5O6S2, which combines PIPES with sodium to enhance solubility and buffering capacity. The sodium sesquihydrate form has a stoichiometric ratio of 1.5 sodium ions per PIPES molecule and provides optimal buffering in the pH range of 6.1 to 7.5, making it ideal for many biological experiments.

PIPES sodium sesquihydrate is often used in cell culture media because it maintains a stable pH, which is essential for cell viability and function, helping to maintain physiological pH and mimic the natural cell environment.

In chromatography, PIPES sodium sesquihydrate serves as an effective buffer during protein purification. Its buffering capacity ensures that proteins remain stable and maintain their activity during separation and analysis.

It is a common component in electrophoresis buffers, which aids in the separation of nucleic acids and proteins by maintaining a stable pH during electrophoresis. This stability is essential for reproducible and accurate results.

PIPES sodium sesquihydrate is used in a variety of biochemical assays, especially those involving enzymes. Its low reactivity with most biochemical reagents ensures that it will not interfere with enzyme activity or assay results.

Unlike some buffers, PIPES sodium sesquihydrate does not significantly chelate metal ions, making it suitable for use in studies involving metal-dependent enzymes. It does not react with common biochemical compounds, reducing the risk of artifacts in experimental results. Its buffering range is suitable for many biological processes, ensuring consistent experimental conditions.

References

1. Synthesis: Goddard, E. D., et al. (1987). "Synthesis and properties of polyquaternium-10." Journal of the Society of Cosmetic Chemists, 38(5), 301-315.

2. Applications: Lochhead, R. Y. (2007). "The use of polyquaternium-10 in hair care formulations." Cosmetics & Toiletries, 122(3), 67-74.

3. Review: Schueller, R., et al. (2010). "Conditioning polymers in personal care: Polyquaternium-10." Allured’s Cosmetics & Toiletries, 125(4), 52-58.