Enzyme secondary antibodies are essential components in immunoassays. These antibodies recognize and bind to primary antibodies, enhancing the detection of specific antigens in various laboratory techniques. Enzymes, like horseradish peroxidase (HRP) or alkaline phosphatase (AP), are typically linked to secondary antibodies. When these secondary antibodies bind to the primary antibody-antigen complex, they facilitate the conversion of substrates into detectable signals, often through colorimetric or chemiluminescent reactions. This amplification step is crucial in assays like ELISAs, enabling the precise quantification and visualization of antigens, making enzyme secondary antibodies a valuable tool in biomedical research and diagnostics.
