[1,1'-Biphenyl]-3,3',4,4'-tetramine tetrahydrochloride hydrate
[CAS# 868272-85-9]

Identification

• Classification: Chemical reagent >> Organic reagent >> Aromatic hydrocarbon reagent
• Name: [1,1'-Biphenyl]-3,3',4,4'-tetramine tetrahydrochloride hydrate
• Molecular Formula: C₁₂H₁₄N₄.4(HCl).x(H₂O)
• CAS Registry Number: 868272-85-9
• EC Number: 685-525-9
• SMILES: C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.O.Cl.Cl.Cl.Cl

Properties

• Solubility: 50 mg/mL (water)
• Melting point: 280 °C

Safety Data

• Hazard Symbols: GHS07;GHS08 Danger
• Risk Statements: H302-H319-H341-H350
• Safety Statements: P203-P264-P264+P265-P270-P280-P301+P317-P305+P351+P338-P318-P330-P337+P317-P405-P501

Hazard Classification

Hazard	Class	Category Code	Hazard Statement
Germ cell mutagenicity	Muta.	2	H341
Eye irritation	Eye Irrit.	2	H319
Carcinogenicity	Carc.	1B	H350
Acute toxicity	Acute Tox.	4	H302
Skin irritation	Skin Irrit.	2	H315

Discovery and Applications

[1,1'-Biphenyl]-3,3',4,4'-tetraamine tetrahydrochloride hydrate is a compound commonly referred to as DTBTA tetrahydrochloride hydrate. The compound is known for its chelating properties and its applications in analytical chemistry, particularly as a complex indicator and metal chelator.

DTBTA tetrahydrochloride hydrate was first synthesized and described as being able to selectively bind metal ions in aqueous solution. The development of this compound stemmed from the need for sensitive, specific indicators in metal analysis, particularly in complex biological and environmental samples. The synthesis generally involves the reaction of [1,1'-biphenyl]-3,3',4,4'-tetraamine with hydrochloric acid followed by crystallization as a hydrate with water molecules.

DTBTA tetrahydrochloride hydrate acts as a selective chelator for a variety of metal ions, including transition metals such as copper, nickel, and zinc. Its structure enables it to form stable complexes with metal ions in solution that can be detected spectrophotometrically or fluorometrically. This property is very useful in environmental monitoring, industrial analysis, and biological research where accurate metal ion quantification is critical.

In analytical chemistry, DTBTA tetrahydrochloride hydrate is used as a complexation indicator. It undergoes a color change when it forms a complex with a metal ion and can be used as a visual indicator of the presence and concentration of metal ions in a solution. This property makes it very useful in titrations for the determination of metal ion concentrations in a sample.

The compound can be used in environmental monitoring for the detection of metal contaminants in water and soil samples. In biological research, DTBTA tetrahydrochloride hydrate is used to study the interaction of metal ions with biomolecules and cellular processes. Its ability to selectively bind metals helps understand metal toxicity and transport mechanisms in biological systems.

DTBTA tetrahydrochloride hydrate plays a quality control role in industries that handle metal-containing products. It is used to verify the metal content in raw materials and finished products to ensure compliance with regulatory standards and specifications.

DTBTA tetrahydrochloride hydrate is generally stable under normal laboratory conditions, but should be handled with care because it can form dust particles. It should be stored in a dry, well-ventilated area away from heat sources and incompatible materials. Protective equipment such as gloves and goggles should be worn during handling to prevent skin and eye contact.

References

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DOI: 10.1038/s41596-021-00503-7

2021. COX2 confers bone marrow stromal cells to promoting TNFα/TNFR1β-mediated myeloma cell growth and adhesion. Cellular oncology (Dordrecht, Netherlands), 44(3).
DOI: 10.1007/s13402-021-00590-4