Triphosphopyridine nucleotide disodium salt
[CAS# 24292-60-2]

Identification

• Classification: Biochemical >> Enzymes and coenzymes
• Name: Triphosphopyridine nucleotide disodium salt
• Synonyms: beta-Nicotinamide adenine dinucleotide phosphate disodium salt; NADP disodium salt
• Molecular Formula: C₂₁H₂₆N₇Na₂O₁₇P₃
• Molecular Weight: 787.37
• CAS Registry Number: 24292-60-2
• EC Number: 246-129-8
• SMILES: C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)([O-])[O-])O)O)O)C(=O)N.[Na+].[Na+]

Properties

• Water solubility: >50 g/L

Safety Data

• Hazard Symbols: GHS07 Warning
• Risk Statements: H302-H315-H319-H335
• Safety Statements: P261-P305+P351+P338

Discovery and Applications

Triphosphopyridine nucleotide disodium salt is a compound that plays a crucial role in the field of biochemistry, especially in processes related to cellular energy transfer and metabolic pathways. It is a derivative of pyridine nucleotide and has three phosphate groups attached to its pyridine ring, contributing to its ability to participate in redox reactions and serve as an essential coenzyme in several biochemical reactions. The disodium salt form of this compound makes it soluble in water, facilitating its use in various biochemical and industrial applications.

The discovery of triphosphopyridine nucleotide disodium salt, as well as its understanding, can be traced back to research on pyridine nucleotides, particularly nicotinamide adenine dinucleotide (NAD) and its phosphorylated derivatives. Researchers identified that the phosphorylated forms of these nucleotides could contribute to energy production and electron transfer within cells. In subsequent years, scientists isolated and studied the triphosphorylated version, recognizing its distinct properties and biological significance.

Triphosphopyridine nucleotide disodium salt is essential for a variety of biochemical applications. It functions as a coenzyme in several oxidation-reduction reactions, playing a role in transferring electrons during metabolic processes such as glycolysis, the citric acid cycle, and oxidative phosphorylation. By participating in these pathways, it helps in the production of ATP, which cells use as a primary energy source. Its ability to carry high-energy electrons makes it indispensable in the bioenergetic processes that sustain life.

In addition to its biological significance, triphosphopyridine nucleotide disodium salt has found application in industrial settings, particularly in the field of biotechnology. It is utilized in enzyme-based processes, where it serves as a cofactor for enzymes involved in the synthesis of important biological molecules. It is also used in research to study redox reactions and cellular metabolism, providing a valuable tool for understanding metabolic diseases, aging, and the effects of oxidative stress.

Another notable application of triphosphopyridine nucleotide disodium salt is in its use in diagnostic and therapeutic research. The compound is employed in assays to measure enzyme activity, such as those involving NAD/NADH or NADP/NADPH systems. Its ability to alter the redox state of the cell makes it useful in studies aimed at understanding the mechanisms of diseases related to energy metabolism, such as cancer and neurodegenerative disorders.

The applications of triphosphopyridine nucleotide disodium salt extend to the pharmaceutical industry as well. Its involvement in cellular energy transfer processes means that it could potentially play a role in drug development aimed at enhancing cellular function or combating diseases where energy metabolism is disrupted. Additionally, its application in enzyme-driven reactions makes it valuable in the production of bio-based chemicals and the development of biocatalysts for sustainable processes.

Ongoing research is focused on expanding the understanding of triphosphopyridine nucleotide disodium salt’s role in cellular metabolism and its potential therapeutic applications. Modifying its structure or introducing it into novel pathways could lead to new biotechnological advancements, especially in the fields of metabolic engineering and synthetic biology.

In summary, triphosphopyridine nucleotide disodium salt is a vital compound in cellular energy processes and redox reactions. Its discovery has significantly advanced our understanding of metabolism, and its applications continue to have a profound impact on both fundamental research and industrial practices, especially in biotechnology and pharmaceuticals.

References

2024. Multispectral Imaging of Collagen, NAD(P)H and Flavin Autofluorescence in Mesenchymal Stem Cells Undergoing Trilineage Differentiation. *Cells*, 13(20).
DOI: 10.3390/cells13201731

1991. Atomic Structure of Ferredoxin-NADP+ Reductase: Prototype for a Structurally Novel Flavoenzyme Family. *Science*, 251(4989).
DOI: 10.1126/science.1986412

1991. Conformation of NADP+ bound to a type II dihydrofolate reductase. *Biochemistry*, 30(6).
DOI: 10.1021/bi00220a003