Online Database of Chemicals from Around the World

2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
[CAS# 6976-37-0]

List of Suppliers
Nanjing Search Biotech Co., Ltd. China Inquire  
+86 (25) 8168-2922
8586-0978
+86 18913919581
trade@searchbio.com.cn
sales@searchbio.com.cn
Linda@searchbio.com.cn
QQ chat
Chemical manufacturer since 2007
chemBlink standard supplier since 2007
Simagchem Corporation China Inquire  
+86 13806087780
sale@simagchem.com
Chemical manufacturer since 2002
chemBlink standard supplier since 2008
Discovery Fine Chemicals Ltd. UK Inquire  
+44 (1202) 874-517
pjc@discofinechem.com
Chemical manufacturer
chemBlink standard supplier since 2009
Wilshire Technologies, Inc. USA Inquire  
+1 (609) 683-1117
Wilshire-info@evonik.com
Chemical manufacturer since 1997
chemBlink standard supplier since 2010
Intatrade Chemicals GmbH Germany Inquire  
+49 (3493) 605-465
sales@intatrade.de
Chemical distributor
chemBlink standard supplier since 2011
Glentham Life Sciences UK Inquire  
+44 (2033) 978-798
info@glenthamls.com
Chemical distributor since 2013
chemBlink standard supplier since 2013
Leap Chem Co., Ltd. China Inquire  
+86 (852) 3060-6658
market19@leapchem.com
QQ chat
Chemical manufacturer since 2006
chemBlink standard supplier since 2015
Shanghai Yuanye Bio-Technology Co., Ltd. China Inquire  
+86 (21) 6184-5781
+86 13585604150
shyysw053@163.com
QQ chat
Chemical manufacturer since 2009
chemBlink standard supplier since 2016
Complete supplier list of 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
Identification
Classification Pharmaceutical intermediate >> Heterocyclic compound intermediate >> Pyrimidine compound >> Alcohol
Name 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
Synonyms Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane; BIS-TRIS
Molecular Structure CAS # 6976-37-0, 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane, BIS-TRIS
Protein Sequence G
Molecular Formula C8H19NO5
Molecular Weight 209.24
CAS Registry Number 6976-37-0
EC Number 230-237-7
SMILES C(CO)N(CCO)C(CO)(CO)CO
Properties
Melting point 103-104 ºC
Solubility 2000 mM (water)
Safety Data
Hazard Symbols symbol symbol   GHS05;GHS07 Danger    Details
Hazard Statements H315-H318-H319-H335    Details
Precautionary Statements P261-P264-P264+P265-P271-P280-P302+P352-P304+P340-P305+P351+P338-P305+P354+P338-P317-P319-P321-P332+P317-P337+P317-P362+P364-P403+P233-P405-P501    Details
Hazard Classification
up    Details
HazardClassCategory CodeHazard Statement
Eye irritationEye Irrit.2H319
Skin irritationSkin Irrit.2H315
Specific target organ toxicity - single exposureSTOT SE3H335
Serious eye damageEye Dam.1H318
Substances or mixtures corrosive to metalsMet. Corr.1H290
Specific target organ toxicity - single exposureSTOT SE3H336
SDS Available
up Discovory and Applicatios
2,2-Bis(hydroxymethyl)-2,2',2''-nitrile triethanol, also known as BIS-TRIS, is an important zwitterionic buffer. It was developed as part of an effort to create a variety of effective biological buffers that met the criteria established by Norman E. Good in the 1960s. Good's buffers were known for their minimal interaction with biological systems, chemical stability, and nontoxicity, making them ideal for a variety of biochemical and biological applications. BIS-TRIS has a stable buffering capacity designed to meet the need for effective control of pH within the physiological range.

The molecular formula of BIS-TRIS is C8H19NO5 and its molecular weight is 209.24 g/mol. Its structure features a tris(hydroxymethyl)aminomethane backbone with additional bis(hydroxymethyl) groups. This structure gives BIS-TRIS excellent buffering properties, making it effective over a pH range of 5.8 to 7.2 and a pKa value of approximately 6.5 at 25°C. This pH range is particularly useful for many biochemical applications, ensuring a stable environment for a variety of reactions and processes.

BIS-TRIS is widely used in electrophoresis buffers to help maintain a consistent pH during protein and nucleic acid separations. Its stable buffering capacity ensures accurate and reproducible results, which is critical for techniques such as SDS-PAGE and agarose gel electrophoresis. Using BIS-TRIS in electrophoresis buffers can improve the resolution and separation of biomolecules.

In cell culture applications, BIS-TRIS helps maintain the pH of the culture medium within the physiological range, promoting optimal cell growth and viability. Its non-toxic properties ensure that it does not interfere with cellular processes, making it a reliable buffer for maintaining consistent experimental conditions in cell-based studies. The stability of BIS-TRIS in cell culture media helps reproducibility of experimental results.

BIS-TRIS is used in a variety of biochemical assays to stabilize pH, which is critical for the activity and stability of enzymes and other biomolecules. Its buffering capacity ensures that assay conditions remain optimal, allowing for accurate and reliable measurements of biochemical activity. The non-interfering properties of BIS-TRIS make it a key ingredient in enzyme research and other biochemical analyses.

During protein purification, maintaining a stable pH is essential to preserve protein structure and function. BIS-TRIS is used in chromatography techniques and other purification protocols to buffer the system and improve the yield and purity of isolated proteins. Its compatibility with different purification methods makes it a versatile tool in protein chemistry.

In structural biology, BIS-TRIS is used in techniques such as X-ray crystallography and NMR spectroscopy to study the structure of proteins and other biomolecules. Its stability and low ionic strength make it an ideal buffer for obtaining high-quality structural data without interfering with the biomacromolecules being analyzed.

The high buffering capacity allows BIS-TRIS to effectively maintain a stable pH, ensuring consistent experimental results. With an appropriate pH range, BIS-TRIS can be used in a variety of experimental conditions, making it a versatile buffer. BIS-TRIS is generally non-toxic to cells and does not interfere with biochemical reactions, making it suitable for sensitive biological applications.

References

Good, N. E., et al. (1966). Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467-477.
Scopes, R. K. (1994). Protein Purification: Principles and Practice. Springer-Verlag.
Righetti, P. G. (2006). Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications. Elsevier.
Market Analysis Reports
List of Reports Available for 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
Related Products
2,2-Bis(hydroxymethyl)-1-azabicyclo[2.2.2]octan-3-one  (2R ,4S)-Rel-2,4-bis(hydroxymethyl)-1-azetidinecarboxylic acid 1,1-dimethylethyl ester  4,4'-Bis(hydroxymethyl)biphenyl  4,4'-Bis(hydroxymethyl)-2,2'-bipyridine  2,2-Bis(hydroxymethyl)butyric acid  2,6-Bis(hydroxymethyl)-p-cresol  1,1-Bis(hydroxymethyl)cyclobutane  1,1-Bis(hydroxymethyl)cyclopropane  3,6-Bis(hydroxymethyl)-1,4-dioxane-2,5-diol  4,4'-Bis(hydroxymethyl)diphenyl ether  N-(1,1-Bis(hydroxymethyl)-3-(4-octylphenyl)propyl)acetamide  9,9-Bis(4-hydroxy-3-methylphenyl)fluorene diglycidyl ether  2,2-Bis(4-hydroxy-3-methylphenyl)propane  Bis(4-hydroxy-3-methylphenyl) sulfide  4,4-Bis(hydroxymethyl)piperidine-1-carboxylic acid tert-butyl ester  2,2-Bis(hydroxymethyl)propionic acid  2,3-Bis(hydroxymethyl)pyridine hydrochloride  2,4-Bis(hydroxymethyl)-1-pyrrolidinecarboxylic acid tert-butyl ester  (2S,4R)-2,4-Bis(hydroxymethyl)-1-pyrrolidinecarboxylic acid tert-butyl ester  2,5-Bis[hydroxymethyl]tetrahydrofuran 


©  chemBlink. All rights reserved.  Content Disclaimer  |  About chemBlink  |  Contact