Stains-all
[CAS# 7423-31-6]

Identification

• Classification: Flavors and spices >> Synthetic spice >> Lactone and oxygen-containing heterocyclic compound >> Thiazole, thiophene and pyridine
• Name: Stains-all
• Synonyms: (2Z)-1-ethyl-2-[(E)-3-(1-ethylbenzo[e][1,3]benzothiazol-1-ium-2-yl)-2-methylprop-2-enylidene]benzo[e][1,3]benzothiazole;bromide
• Molecular Formula: C₃₀H₂₇N₂S₂ ^.Br
• Molecular Weight: 559.58
• CAS Registry Number: 7423-31-6
• EC Number: 231-047-7
• SMILES: CCN1/C(=C/C(=C/C2=[N+](C3=C(S2)C=CC4=CC=CC=C43)CC)/C)/SC5=C1C6=CC=CC=C6C=C5.[Br-]

Properties

• Solubility: 40 mg (4.0 mL Chloroform)

Discovory and Applicatios

Stains-all is a synthetic dye primarily known for its ability to stain nucleic acids, proteins, and other biological molecules. This chemical compound, whose IUPAC name is 3-(2-methyl-3-benzothiazolyl)-7-(3-sulfopropyl)-2H-1-benzothiopyran-2-one, is widely used in biochemical and molecular biology research. The compound is a member of the class of dyes known as thiazole dyes, which are characterized by their vivid colors and ability to form stable complexes with biomolecules.

The discovery of Stains-all dates back to the early research efforts aimed at improving the specificity and sensitivity of staining techniques used in laboratories. Researchers were interested in developing a versatile dye capable of binding to a wide variety of biomolecules, particularly nucleic acids and proteins, without the need for harsh chemical treatments. Stains-all was found to exhibit unique binding properties that allowed it to selectively stain these biomolecules under mild conditions. It has since become a staple in the field of molecular biology for various staining protocols.

Stains-all is used primarily for the detection and visualization of nucleic acids, such as DNA and RNA, and proteins in electrophoresis gels. It is particularly useful for applications involving agarose or polyacrylamide gel electrophoresis, where the separation of biomolecules based on size is necessary. The dye binds to nucleic acids and proteins, forming complexes that can be detected using fluorescence or UV light. The unique properties of Stains-all enable it to produce sharp, clear bands, even in gels that contain a mixture of biomolecules, making it a versatile tool in laboratory settings.

In addition to its use in electrophoresis, Stains-all has applications in protein and nucleic acid quantification. It is used in assays that require the detection of specific proteins or DNA fragments in complex biological samples. The dye's ability to produce strong fluorescent signals when bound to nucleic acids and proteins allows for high sensitivity in detecting low-abundance molecules, which is crucial in research areas such as gene expression analysis, protein analysis, and diagnostics.

Moreover, Stains-all has been explored for its potential in cell biology. It is used for labeling and visualizing cellular components, particularly in fluorescence microscopy. The dye's ability to selectively bind to nucleic acids and proteins within live cells or tissues makes it a valuable tool for researchers studying cell morphology, gene expression, and protein localization.

One of the notable advantages of Stains-all over other traditional dyes is its ability to bind to both nucleic acids and proteins with minimal interference. While other dyes may require specific conditions or treatments for nucleic acids and proteins, Stains-all’s broad binding profile simplifies protocols and reduces the need for multiple reagents, making it cost-effective and time-efficient for researchers.

In summary, Stains-all is a highly effective and versatile dye used in various biochemical applications, including nucleic acid and protein staining, electrophoresis, and cell biology. Its discovery revolutionized the way researchers visualize and quantify biomolecules, contributing significantly to molecular biology research.